HYGEIA Hospital
ΜΗΤΕΡΑ
Παίδων ΜΗΤΕΡΑ
ΛΗΤΩ Μαιευτικό, Γυναικολογικό & Χειρουργικό Κέντρο
HYGEIA Hospital Tirana
Y-Logimed Α.Ε.
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The Cytopathology Department of HYGEIA, one of the leading laboratories in our country applies current state-of-the-art methods and features high precision equipment of latest technology.

The laboratory can perform all cytological tests regarding every system of the human body, aiming at prevention, early diagnosis and follow-up of malignant and non-malignant diseases.

Charitini Salla , M.D,M.I.A.C leads the Department which comprises of highly experienced and skilled doctors and cytotechnologists. The whole team keeps abreast of the newest developments on the field and attends national and international congresses, training seminars on an on-going and systematic basis. Our doctors have also published research papers in several Greek and international medical journals.

 

 

 

CONFERENCES

16/1/2016: “Cytology in the Past, the Present and the Future: From Clinical to Molecular Cytology

11/3/2017: “2nd Scientific Meeting Zebras and Unicorns in cytology Interesting cases

 

1. PREVENTION

Prevention and cytology are fully interconnected, since the most effective preventive test in medicine the internationally-known test Papanicolaou, is cytological.

The most modern scientific techniques are applied in the Cytopathology Laboratory in the whole spectrum of cytological materials , that is liquid based cytology, cytochemistry, immunocytochemistry, hormonal receptors and other prognostic indices. Further more , FISH (immunofluorescence in situ hybridisation )for the detection of mutated genes and HPV-DNA typing is possible on cytological material in collaboration with the pathology and molecular biology departments of HYGEIA hospital.
These techniques require technological equipment of the highest standards; special training of the medical and technical personnel and have high clinical significance, since they offer the possibility of a more accurate diagnosis, prognosis and treatment.

  • Liquid Based Cytology (Thin Prep): the most modern method of cytological samples processing, the liquid phase cytology (Thin Prep), is applied in the Cytopathology Laboratory. This method is internationally recognized as one contributing in the increase of cytological tests reliability, in all cytological materials. Cells are collected in a vial containing preservation fluid, which is placed in a special apparatus and a monolayer smear is automatically created. The rest of the vial’s material is available for performing further diagnostic techniques, which might be required for clarifying or confirming the diagnosis.
    Particularly for the Pap test , the monolayer smear of cervical cells, free of blood and inflammatory cells, makes even the smallest number of pathological cells visible that could have otherwise gone unnoticed using the conventional method, thus resulting in the reduction of false-negative answers and the increase of the Pap test diagnostic accuracy. Moreover, the material remaining in the vial, can be used for additional tests, without obtaining a new sample (eg. molecular techniques for HPV detection and typing, when the result of the Pap test provides such an indication).
  • Cytochemistry and immunocytochemistry for the identification and typing of the neoplasms 
  • Immunocytochemical determination of estrogen and progesterone receptors and oncogene HER-2/neu expression in FNA of breast cancer.
  • Nuclear grading of malignancy.

4. SECOND OPINION CONSULTATION SERVICES

The Cytopathology Department accepts any patient who already has his/her cytological slides examined/diagnosed, for a second opinion. The patient is obliged to bring the following documents:

  • Cytological smears( both conventional and liquid based )as well as cell block preparations (paraffin embedded material)
  • Slides of the various cyto/immunocytochemical stains ,that have already been performed.
  • Paraffin cube/s(cell block material) for further cyto/immunocytochemical studies if deemed necessary.
  • Copy of the rendered cytological diagnosis.

Obtained material is recorded in detail on a document and titled “second opinion on material accepted from another hospital”.

The material will be returned to the patient upon completion of the examination while any cyto/immunocytochemical slides from HYGEIAS lab will be kept in our archives.

*The material and especially the fragile cytological slides must be packed with great caution in the special boxes, cytology labs usually have. The material does not need to be preserved in a fridge and can be left at room temperature until we receive it.

5. CONSULTATION SERVICES

Experienced staff of the Laboratory [authorized scientists who meet the requirements of ISO standard 15189 (5.1.2 & 5.1.6) communicate with the users of the services the laboratory provides, according to the following rules and regulations established by the Committee of the clinical laboratories of HYGEIA

  • The clinical doctor treating each patient chooses the examinations to be carried out 
  • The laboratory provides appropriate information regarding the necessity of carrying out selected exams, relevant to the therapeutic options
  • It provides additional explanatory notes on the result of an examination
  • It provides information to the clinical doctor as regards the necessity and the usefulness of a specific additional examination
  • It provides instructions about preparing the patient for the sample collection as well as the limitations of the requested examination
  • It proposes the most useful examination for diagnosis and treatment and the material that should be obtained for it.
  • The laboratory informs the physicians, for any deviation in the criteria of accepted samples, which might affect the analytical phase of the examination
  • It also proposes further tests on the same sample, using a different methodology for confirming the result

6. CYTOLOGICAL SAMPLES THROUGH THE POST

The Cytopathology department accepts cytological material from any part of Greece, subsequent to a communication of the sender with the department for ensuring the proper delivery of the samples, following the suggested procedures.

The cytopathology laboratory does not accept:

  • Cytological material without a referral, a doctor’s note and proper identification of the patient
  • Slides or paraffin embedded material (cell block) broken or tampered or without the corresponding cytological report, when it comes to an advisory opinion
  • Histopathology slides, unless for comparison purposes with the material of the micro biopsy, (cell block)
  • Blood and blood derivatives
  • The laboratory does not accept blood samples for detection of circulating cancer cells.

7. TURNAROUND TIME (Time frame in working days)

  • CONVENTIONAL PAP TEST: 5 DAYS 
  • LIQUID BASED PAP TEST: 2 DAYS
  • BODY FLUID SAMPLE : 3 DAYS 
  • CEREBROSPINAL FLUID SAMPLE: 2 DAYS
  • BRONCHOALVEOLAR LAVAGE (BAL): 3 DAYS 
  • SPECIMENS COLLECTED WITH A BRUSH: 3 DAYS
  • FNA OF PALPATED AND DEEP –SEATED ORGANS: 2 -10 DAYS 
  • CYTO/IMMUNOCYTOCHEMICAL EXAMINATION: 5 DAYS
  • BRONCHIAL WASHINGS-BRUSHINGS

Probable prolongation of the turnaround time may rarely occur in case of immunocytochemical investigation of a metastatic malignant neoplasm of unknown primary site.

8. PARTICIPATION IN MULTIDESCIPLINARY MEETINGS

The cytopathology laboratory participates in the oncological meetings of the hospital, whereas challenging cases are presented in detail. Doctors comment on each case, based on their field of expertise.

  • Breast cancer oncological meetings- V.kyriakidou
  • Digestive system cancer oncological meetings: Ch. Salla
  • Gynecologic cancer oncological meetings- A.Kapoula).

9. INTERNATIONAL COLLABORATION:

The Hygeia cytopathology laboratory collaborates with internationally renowned laboratories such as:

  • Massachusetts General hospital /Harvard University ( Prof Martha Bishop Pitman ).
  • Weil Cornell Medical college, ( Grace Yang).

Level A of MITERA Hospital

Telephone
HYGEIA
+30 210 686 7458
MITERA
+30 210 686 9834-811